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aav1 ef1a flp  (Addgene inc)


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    Addgene inc aav1 ef1a flp
    Aav1 Ef1a Flp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav1+ef1a+flp/pAAV-EF1a-Flpo+(Plasmid+%2355637)/10__1523_slash_jneurosci__1626___25__2025-58-22-23
    Average 95 stars, based on 84 article reviews
    aav1 ef1a flp - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Injection:

    Article Title: A distinct circuit for biasing visual perceptual decisions and modulating superior colliculus activity through the mouse posterior striatum
    Article Snippet: .. For di-synaptic tracing of DMS and PS direct pathways through the SN in wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected in the DMS and the other virus in the PS (same volumes and coordinates as listed above), as well as Fluorogold (30 nl, 1.5%, Fluorochrome LLC, Denver, CO) to label the injection site. .. In the same mice, both AAV1-Ef1a-fDIO-mCherry (Addgene #114471-AAV1) and AAV1-Ef1a-DIO-eYFP (Addgene #27056-AAV1) were injected in the SNR (150nl each; [AP] -3.2, [ML] 1.6, [DV] -4.4).

    Article Title: Prefrontal feature representations drive memory recall.
    Article Snippet: Memory formation involves binding of contextual features into a unitary representation, whereas memory recall can occur using partial combinations of these contextual features.. The neural basis underlying the relationship between a contextual memory and its constituent features is not well understood; in particular, where features are represented in the brain and how they drive recall.. Here, to gain insight into this question, we developed a behavioural task in which mice use features to recall an associated contextual memory.

    Article Title: A distinct circuit for biasing visual perceptual decisions and modulating superior colliculus activity through the mouse posterior striatum
    Article Snippet: .. 148 For di-synaptic tracing of DMS and PS direct pathways through the SN in wild-type mice, 149 either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene 150 #55637-AAV1) was unilaterally injected in the DMS and the other virus in the PS (same 151 volumes and coordinates as listed above), as well as Fluorogold (30 nl, 1.5%, Fluorochrome 152 LLC, Denver, CO) to label the injection site. .. In the same mice, both AAV1-Ef1a-fDIO-mCherry 153 (Addgene #114471-AAV1) and AAV1-Ef1a-DIO-eYFP (Addgene #27056-AAV1) were injected in 154 the SNR (150nl each; [AP] -3.2, [ML] 1.6, [DV] -4.4).

    Article Title: A Distinct Circuit for Biasing Visual Perceptual Decisions and Modulating Superior Colliculus Activity through the Mouse Posterior Striatum
    Article Snippet: .. For disynaptic tracing of DMS and PS direct pathways through the SN in wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected into the DMS, with the other virus injected into the PS (same volumes and coordinates as listed above), as well as Fluoro-Gold (30 nl, 1.5%; Fluorochrome) to label the injection site. .. In the same mice, both AAV1-Ef1a-fDIO-mCherry (Addgene #114471-AAV1) and AAV1-Ef1a-DIO-eYFP (Addgene #27056-AAV1) were injected into the SNR [150 nl each; (AP) −3.2, (ML) 1.6, (DV) −4.4].

    Virus:

    Article Title: A distinct circuit for biasing visual perceptual decisions and modulating superior colliculus activity through the mouse posterior striatum
    Article Snippet: .. For di-synaptic tracing of DMS and PS direct pathways through the SN in wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected in the DMS and the other virus in the PS (same volumes and coordinates as listed above), as well as Fluorogold (30 nl, 1.5%, Fluorochrome LLC, Denver, CO) to label the injection site. .. In the same mice, both AAV1-Ef1a-fDIO-mCherry (Addgene #114471-AAV1) and AAV1-Ef1a-DIO-eYFP (Addgene #27056-AAV1) were injected in the SNR (150nl each; [AP] -3.2, [ML] 1.6, [DV] -4.4).

    Article Title: A distinct circuit for biasing visual perceptual decisions and modulating superior colliculus activity through the mouse posterior striatum
    Article Snippet: .. 148 For di-synaptic tracing of DMS and PS direct pathways through the SN in wild-type mice, 149 either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene 150 #55637-AAV1) was unilaterally injected in the DMS and the other virus in the PS (same 151 volumes and coordinates as listed above), as well as Fluorogold (30 nl, 1.5%, Fluorochrome 152 LLC, Denver, CO) to label the injection site. .. In the same mice, both AAV1-Ef1a-fDIO-mCherry 153 (Addgene #114471-AAV1) and AAV1-Ef1a-DIO-eYFP (Addgene #27056-AAV1) were injected in 154 the SNR (150nl each; [AP] -3.2, [ML] 1.6, [DV] -4.4).

    Article Title: A Distinct Circuit for Biasing Visual Perceptual Decisions and Modulating Superior Colliculus Activity through the Mouse Posterior Striatum
    Article Snippet: .. For disynaptic tracing of DMS and PS direct pathways through the SN in wild-type mice, either AAV1-hSyn-cre-WPRE-hGHpA (Addgene #105553-AAV1) or AAV1-Ef1a-flp (Addgene #55637-AAV1) was unilaterally injected into the DMS, with the other virus injected into the PS (same volumes and coordinates as listed above), as well as Fluoro-Gold (30 nl, 1.5%; Fluorochrome) to label the injection site. .. In the same mice, both AAV1-Ef1a-fDIO-mCherry (Addgene #114471-AAV1) and AAV1-Ef1a-DIO-eYFP (Addgene #27056-AAV1) were injected into the SNR [150 nl each; (AP) −3.2, (ML) 1.6, (DV) −4.4].

    Anterograde Tracing:

    Article Title: Prefrontal feature representations drive memory recall.
    Article Snippet: Memory formation involves binding of contextual features into a unitary representation, whereas memory recall can occur using partial combinations of these contextual features.. The neural basis underlying the relationship between a contextual memory and its constituent features is not well understood; in particular, where features are represented in the brain and how they drive recall.. Here, to gain insight into this question, we developed a behavioural task in which mice use features to recall an associated contextual memory.



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    a, Schematic for BNSTvGAT→PVH neuron CRACM (n = 18 PVHCrh+ neurons, n = 9 PVHCrh− neurons/2 mice). b, Representative traces from BNSTvGAT→PVHCrh+ (upper) and BNSTvGAT→PVHCrh− (lower) recordings. c, Schematic of BNST or LH injection of Cre-dependent AAV-hM4Di-mCherry into vGAT-IRES-Cre mice. d, Time-line of chemogenetic inhibition of BNSTvGAT or LHvGAT neurons. e, Inhibition of BNSTvGat neurons increased corticosterone (BNST: n = 4 vGATmCherry, n = 4 vGAThM4Di. Two-tailed unpaired t-test- *P = 0.0131; LH: n = 5 vGATmCherry, n = 7 vGAThM4Di. Two-tailed unpaired t-test- P = 0.2837). f, Schematic of BNSTvGAT→PVH terminal inhibition. g, Representative eOpn3 expression in the BNST (upper) and location of optic fibers in the <t>PVH</t> (lower). h, Time-line for terminal inhibition of BNSTvGAT→PVH projections. i, Inhibition of BNSTvGAT→PVH projections increased corticosterone (n = 3 vGATmCherry, n = 5 vGATeOpn3. Two-tailed unpaired t-test- *P = 0.0076). j, Schematic of BNST→PVH terminal recordings with optogenetic stimulation of AgRP→PVH terminals. k, Representative images of synaptophysin-GCaMP6f expression in BNST neurons and terminals and ChrimsonR-tdTomato expression in AgRP neuron terminals. l, Heatmaps of BNST→PVH fiber photometry recordings from two mice. m, n, Optogenetic stimulation of AgRP→PVH terminals for 10 sec at 20 Hz (m) (n = 3 mice) and 10 min at 20 Hz (n) (n = 4 mice) reduced activity of BNST→PVH terminals. o, Representative images <t>of</t> <t>BFP-labelled</t> PVHCrh neurons, synaptophysin-GCaMP6f-expressing BNST neuron terminals and AgRP immunofluorescence (* = PVHCrh neurons apposed by BNST and AgRP neuron terminals). p, Schematic of AgRP neuron action in the PVH. Left = presynaptic disinhibition of PVHCrh neurons by GABA/presynaptic GABA-B-Rs. Right = monosynaptic inhibition of PVHMc4r neurons by GABA/postsynaptic GABA-A-Rs. Scale bar (g, k) = 200 μm. Scale bar (o) = 10 μm. Data represent = mean ± sem.
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    a, Schematic for BNSTvGAT→PVH neuron CRACM (n = 18 PVHCrh+ neurons, n = 9 PVHCrh− neurons/2 mice). b, Representative traces from BNSTvGAT→PVHCrh+ (upper) and BNSTvGAT→PVHCrh− (lower) recordings. c, Schematic of BNST or LH injection of Cre-dependent AAV-hM4Di-mCherry into vGAT-IRES-Cre mice. d, Time-line of chemogenetic inhibition of BNSTvGAT or LHvGAT neurons. e, Inhibition of BNSTvGat neurons increased corticosterone (BNST: n = 4 vGATmCherry, n = 4 vGAThM4Di. Two-tailed unpaired t-test- *P = 0.0131; LH: n = 5 vGATmCherry, n = 7 vGAThM4Di. Two-tailed unpaired t-test- P = 0.2837). f, Schematic of BNSTvGAT→PVH terminal inhibition. g, Representative eOpn3 expression in the BNST (upper) and location of optic fibers in the <t>PVH</t> (lower). h, Time-line for terminal inhibition of BNSTvGAT→PVH projections. i, Inhibition of BNSTvGAT→PVH projections increased corticosterone (n = 3 vGATmCherry, n = 5 vGATeOpn3. Two-tailed unpaired t-test- *P = 0.0076). j, Schematic of BNST→PVH terminal recordings with optogenetic stimulation of AgRP→PVH terminals. k, Representative images of synaptophysin-GCaMP6f expression in BNST neurons and terminals and ChrimsonR-tdTomato expression in AgRP neuron terminals. l, Heatmaps of BNST→PVH fiber photometry recordings from two mice. m, n, Optogenetic stimulation of AgRP→PVH terminals for 10 sec at 20 Hz (m) (n = 3 mice) and 10 min at 20 Hz (n) (n = 4 mice) reduced activity of BNST→PVH terminals. o, Representative images <t>of</t> <t>BFP-labelled</t> PVHCrh neurons, synaptophysin-GCaMP6f-expressing BNST neuron terminals and AgRP immunofluorescence (* = PVHCrh neurons apposed by BNST and AgRP neuron terminals). p, Schematic of AgRP neuron action in the PVH. Left = presynaptic disinhibition of PVHCrh neurons by GABA/presynaptic GABA-B-Rs. Right = monosynaptic inhibition of PVHMc4r neurons by GABA/postsynaptic GABA-A-Rs. Scale bar (g, k) = 200 μm. Scale bar (o) = 10 μm. Data represent = mean ± sem.
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    a, Schematic for BNSTvGAT→PVH neuron CRACM (n = 18 PVHCrh+ neurons, n = 9 PVHCrh− neurons/2 mice). b, Representative traces from BNSTvGAT→PVHCrh+ (upper) and BNSTvGAT→PVHCrh− (lower) recordings. c, Schematic of BNST or LH injection of Cre-dependent AAV-hM4Di-mCherry into vGAT-IRES-Cre mice. d, Time-line of chemogenetic inhibition of BNSTvGAT or LHvGAT neurons. e, Inhibition of BNSTvGat neurons increased corticosterone (BNST: n = 4 vGATmCherry, n = 4 vGAThM4Di. Two-tailed unpaired t-test- *P = 0.0131; LH: n = 5 vGATmCherry, n = 7 vGAThM4Di. Two-tailed unpaired t-test- P = 0.2837). f, Schematic of BNSTvGAT→PVH terminal inhibition. g, Representative eOpn3 expression in the BNST (upper) and location of optic fibers in the <t>PVH</t> (lower). h, Time-line for terminal inhibition of BNSTvGAT→PVH projections. i, Inhibition of BNSTvGAT→PVH projections increased corticosterone (n = 3 vGATmCherry, n = 5 vGATeOpn3. Two-tailed unpaired t-test- *P = 0.0076). j, Schematic of BNST→PVH terminal recordings with optogenetic stimulation of AgRP→PVH terminals. k, Representative images of synaptophysin-GCaMP6f expression in BNST neurons and terminals and ChrimsonR-tdTomato expression in AgRP neuron terminals. l, Heatmaps of BNST→PVH fiber photometry recordings from two mice. m, n, Optogenetic stimulation of AgRP→PVH terminals for 10 sec at 20 Hz (m) (n = 3 mice) and 10 min at 20 Hz (n) (n = 4 mice) reduced activity of BNST→PVH terminals. o, Representative images <t>of</t> <t>BFP-labelled</t> PVHCrh neurons, synaptophysin-GCaMP6f-expressing BNST neuron terminals and AgRP immunofluorescence (* = PVHCrh neurons apposed by BNST and AgRP neuron terminals). p, Schematic of AgRP neuron action in the PVH. Left = presynaptic disinhibition of PVHCrh neurons by GABA/presynaptic GABA-B-Rs. Right = monosynaptic inhibition of PVHMc4r neurons by GABA/postsynaptic GABA-A-Rs. Scale bar (g, k) = 200 μm. Scale bar (o) = 10 μm. Data represent = mean ± sem.
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    Image Search Results


    a, Schematic for BNSTvGAT→PVH neuron CRACM (n = 18 PVHCrh+ neurons, n = 9 PVHCrh− neurons/2 mice). b, Representative traces from BNSTvGAT→PVHCrh+ (upper) and BNSTvGAT→PVHCrh− (lower) recordings. c, Schematic of BNST or LH injection of Cre-dependent AAV-hM4Di-mCherry into vGAT-IRES-Cre mice. d, Time-line of chemogenetic inhibition of BNSTvGAT or LHvGAT neurons. e, Inhibition of BNSTvGat neurons increased corticosterone (BNST: n = 4 vGATmCherry, n = 4 vGAThM4Di. Two-tailed unpaired t-test- *P = 0.0131; LH: n = 5 vGATmCherry, n = 7 vGAThM4Di. Two-tailed unpaired t-test- P = 0.2837). f, Schematic of BNSTvGAT→PVH terminal inhibition. g, Representative eOpn3 expression in the BNST (upper) and location of optic fibers in the PVH (lower). h, Time-line for terminal inhibition of BNSTvGAT→PVH projections. i, Inhibition of BNSTvGAT→PVH projections increased corticosterone (n = 3 vGATmCherry, n = 5 vGATeOpn3. Two-tailed unpaired t-test- *P = 0.0076). j, Schematic of BNST→PVH terminal recordings with optogenetic stimulation of AgRP→PVH terminals. k, Representative images of synaptophysin-GCaMP6f expression in BNST neurons and terminals and ChrimsonR-tdTomato expression in AgRP neuron terminals. l, Heatmaps of BNST→PVH fiber photometry recordings from two mice. m, n, Optogenetic stimulation of AgRP→PVH terminals for 10 sec at 20 Hz (m) (n = 3 mice) and 10 min at 20 Hz (n) (n = 4 mice) reduced activity of BNST→PVH terminals. o, Representative images of BFP-labelled PVHCrh neurons, synaptophysin-GCaMP6f-expressing BNST neuron terminals and AgRP immunofluorescence (* = PVHCrh neurons apposed by BNST and AgRP neuron terminals). p, Schematic of AgRP neuron action in the PVH. Left = presynaptic disinhibition of PVHCrh neurons by GABA/presynaptic GABA-B-Rs. Right = monosynaptic inhibition of PVHMc4r neurons by GABA/postsynaptic GABA-A-Rs. Scale bar (g, k) = 200 μm. Scale bar (o) = 10 μm. Data represent = mean ± sem.

    Journal: Nature

    Article Title: Neural basis for fasting activation of hypothalamic-pituitary-adrenal axis

    doi: 10.1038/s41586-023-06358-0

    Figure Lengend Snippet: a, Schematic for BNSTvGAT→PVH neuron CRACM (n = 18 PVHCrh+ neurons, n = 9 PVHCrh− neurons/2 mice). b, Representative traces from BNSTvGAT→PVHCrh+ (upper) and BNSTvGAT→PVHCrh− (lower) recordings. c, Schematic of BNST or LH injection of Cre-dependent AAV-hM4Di-mCherry into vGAT-IRES-Cre mice. d, Time-line of chemogenetic inhibition of BNSTvGAT or LHvGAT neurons. e, Inhibition of BNSTvGat neurons increased corticosterone (BNST: n = 4 vGATmCherry, n = 4 vGAThM4Di. Two-tailed unpaired t-test- *P = 0.0131; LH: n = 5 vGATmCherry, n = 7 vGAThM4Di. Two-tailed unpaired t-test- P = 0.2837). f, Schematic of BNSTvGAT→PVH terminal inhibition. g, Representative eOpn3 expression in the BNST (upper) and location of optic fibers in the PVH (lower). h, Time-line for terminal inhibition of BNSTvGAT→PVH projections. i, Inhibition of BNSTvGAT→PVH projections increased corticosterone (n = 3 vGATmCherry, n = 5 vGATeOpn3. Two-tailed unpaired t-test- *P = 0.0076). j, Schematic of BNST→PVH terminal recordings with optogenetic stimulation of AgRP→PVH terminals. k, Representative images of synaptophysin-GCaMP6f expression in BNST neurons and terminals and ChrimsonR-tdTomato expression in AgRP neuron terminals. l, Heatmaps of BNST→PVH fiber photometry recordings from two mice. m, n, Optogenetic stimulation of AgRP→PVH terminals for 10 sec at 20 Hz (m) (n = 3 mice) and 10 min at 20 Hz (n) (n = 4 mice) reduced activity of BNST→PVH terminals. o, Representative images of BFP-labelled PVHCrh neurons, synaptophysin-GCaMP6f-expressing BNST neuron terminals and AgRP immunofluorescence (* = PVHCrh neurons apposed by BNST and AgRP neuron terminals). p, Schematic of AgRP neuron action in the PVH. Left = presynaptic disinhibition of PVHCrh neurons by GABA/presynaptic GABA-B-Rs. Right = monosynaptic inhibition of PVHMc4r neurons by GABA/postsynaptic GABA-A-Rs. Scale bar (g, k) = 200 μm. Scale bar (o) = 10 μm. Data represent = mean ± sem.

    Article Snippet: To label PVH Crh neuron somas, AAV1-Ef1a-Cre(ON)/Flp(OFF) 2.0-BFP (Addgene 137130-AAV1; 2.24 × 10 13 viral genomes ml −1 ) was injected into the PVH of Crh- IRES-Cre mice.

    Techniques: Injection, Inhibition, Two Tailed Test, Expressing, Activity Assay, Immunofluorescence